Electrophysiological effects of extracellular ATP on Necturus gallbladder epithelium.
نویسندگان
چکیده
منابع مشابه
Electrophysiological Effects of Extracellular ATP on Necturus Epithelium Gallbladder
The effects of addit ion of ATP to the mucosal bathing solution on transepithelial, apical, and basolateral membrane voltages and resistances in Necturus gal lbladder epithelium were determined. Mucosal ATP (100 IxM) caused a rapid hyperpolarization of both apical (Vmc) and basolateral (Vcs) cell membrane voltages (AV m -18 -+ 1 mV), a fall in transepithelial resistance (Rt) from 142 -+ 8 to 12...
متن کاملElectrophysiological effects of extracellular ATP on Necturus gallbladder epithelium
The effects of addition of ATP to the mucosal bathing solution on transepithelial, apical, and basolateral membrane voltages and resistances in Necturus gallbladder epithelium were determined. Mucosal ATP (100 microM) caused a rapid hyperpolarization of both apical (Vmc) and basolateral (Vcs) cell membrane voltages (delta Vm = 18 +/- 1 mV), a fall in transepithelial resistance (Rt) from 142 +/-...
متن کاملElectrophysiological effects of basolateral [Na+] in Necturus gallbladder epithelium
In Necturus gallbladder epithelium, lowering serosal [Na+] ([Na+]s) reversibly hyperpolarized the basolateral cell membrane voltage (Vcs) and reduced the fractional resistance of the apical membrane (fRa). Previous results have suggested that there is no sizable basolateral Na+ conductance and that there are apical Ca(2+)-activated K+ channels. Here, we studied the mechanisms of the electrophys...
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Transepithelial fluid transport(/,) and intracellular Na' and CIactivities (aNai , aCl;) were measured in isolated Necturus gallbladders to establish the contribution of different proposed apical membrane entry mechanisms to transepithelial salt transport . In 10 mM HC03-Ringer's, Jv was 13.5 ± 1 .1 ttl cm-'-h, 'and was significantly reduced by a low bicarbonate medium and by addition of amilor...
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An electrophysiological technique that is sensitive to small changes in cell water content and has good temporal resolution was used to determine the hydraulic permeability (Lp) of Necturus gallbladder epithelium. The epithelial cells were loaded with the impermeant cation tetramethylammonium (TMA+) by transient exposure to the pore-forming ionophore nystatin in the presence of bathing solution...
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ژورنال
عنوان ژورنال: Journal of General Physiology
سال: 1991
ISSN: 0022-1295,1540-7748
DOI: 10.1085/jgp.97.5.949